Description: Recently upgraded confocal microscope using the more conventional concepts of mirrors and filters to select for specific wavelengths

Laser: Krypton/Argon

Laser lines: 488, 568 and 647nm

Microscope: Zeiss Axioskop - upright

Objectives:
2.5X Plan-NEOFLUAR NA 0.075 - dry
10X Plan-NEOFLUAR NA 0.3 - dry
25X Plan-NEOFLUAR NA 0.8 - water
40X C-APOCHROMAT NA 1.2 - water
63X C-APOCHROMAT NA 1.2 - water

Software platform: LaserSharp 2000

Software capabilities: triple channel scanning (simultaneous or sequential scanning) plus transmitted light, time series, Z-series, several 3D projection modes (3D reconstruction, max brightness, average, threshold, and etc)

File format: PIC (export capabilities to TIF, JPG, AVI movies)

Compatible fluorophores: GFP, Bodipy, FITC, Alexa 488, YFP, Lucifer Yellow, CY3, TRITC, dsRed, Alexa 568, Texas Red, Propidium Iodide, CY5

Description: This system uses state-of-the-art confocal technology such as the acousto-optical beam splitter to select specific wavelengths. Equipped with 4 different lasers, this system is completely tunable and compatible with virtually all fluorophores

Lasers: UV, Argon / Argon Krypton, Helium Neon 543 / 594 and Helium Neon 633

Laser lines: 405, 458, 476, 488, 496, 514, 543, 594 and 633nm

Microscope: Leica DM IRE2 - Inverted, set up for Phase and Nomarski optics

Objectives:
10X HC PL APO NA 0.4 - dry
20X HC PL APO NA 0.7 - dry
63X HCX PL APO NA 1.2 - water
63X HCX PL APO NA 1.4 - oil

Software platform: Leica Confocal Software (LCS)

Software capabilities: quadruple channel scanning (simultaneous or sequential) plus transmitted light, spectral un-mixing of signals, spectral scanning, time series, Z-series, 3D projections and reconstructions, stereo animations and projections, quantification (generates statistical reports, histograms, and etc)

System applications: FRAP, FLIP, FRET, spectral scans

File format: TIF, export capabilities to AVI movie

Description: This microscope system uses a focused cold laser beam to excise portions of a tissue sample or section. These cut sections which can be as small as one to several cells, are then laser catapulted from the sample into a collection tube for RNA or protein for metabolite analysis.

Description: This is a wide field compound (upright) microscope set up to do high quality Normarski, Phase Contrast, and Polarization Microscopy. This microscope is also set up to do epifluorescence

Filter sets: DAPI, FITC, TRITC

Description: This setup is a dissecting microscope, equipped with a fluorescence light source, which allows for quick screening for plants with fluorescent gene expression

Objectives:
0.5X HR PlanApo WD 136
1X HR PlanApo WD 54
1.6X HR PlanApo WD 24

Description: This setup is a dissecting microscope, equipped with fluorescence, which allows for quick screening for plant with fluorescent gene expression

Objectives:
1X PF DF PLAPO
1.6X PF DF PLAPO

Description: Compound, upright microscope for standard wide field applications. This microscope is also set up to do epifluorescence

Objectives:
4X Plan NA 0.13
10X Plan NA 0.3
20X PlanFluor NA 0.5
40X Fluor NA 1.3
60X M Plan NA 0.7

Filter sets: DAPI, FITC, TRITC

Description: This set up facilitates wide field time lapse imaging of live cells in transmitted light as well as fluorescence. The Princeton Instruments camera with its high dynamic range allows ratiometric and quantitative ion imaging.

Microscope: Nikon Eclipse TE 300 - Inverted

Objectives:
10X PlanFluor NA 0.3 Ph1 DLL - dry
20X PlanFluor NA 0.5 DIC M - dry
40X PlanFluor NA 1.3 DIC H - oil
100X PlanFluor NA 1.3 DIC H - oil

Cameras:
Leica DC 200 (for time lapse)
Princeton Instruments (high dynamic range for quantitative fluorescence and ratiometric imaging)

Software platform: Metamorph and Metafluor

Software capabilities: time lapse, fluorescence quantification, track objects, area-line measurements

System applications: time lapse (transmitted light and epifluorescence), ratiometric ion imaging

Microtome for 20-100μm thickness

Microtome for 20-100μm thickness

Freezing microtome for 10-60μm thickness

Microtome for 0.5-100μm thickness

Ultra microtome for both light and transmission electron microscope

Ultra microtome for both light and transmission electron microscope