| The Sumner Group: Protocols |
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Protocols
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In-gel Digestion of Coomassie Stained Proteins (No Reduction/Alkylation) |
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In-gel Digestion of Coomassie Stained Proteins (with DTT Reduction and Iodoacetaminde Alkylation) |
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In-gel Digestion of Silver Stained Proteins (with DTT Reduction and Iodoacetaminde Alkylation) |
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Silver Destaining Using Hydrogen Peroxide |
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TCA/Acetone Protein Extraction |
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TRIS Extraction of Cells |
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Metabolite Extraction and Derivatization of NSF-Funded Cell Culture Tissue using a Bi-Phasic Solvent System Tissue preparation and extraction Preparation and Derivatization of polar metabolites Preparation and Derivatization of non-polar metabolites |
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